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KMID : 0882420060710010010
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Korean Journal of Medicine
2006 Volume.71 No. 1 p.10 ~ p.16
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Identification of Brucella abortus using the sequencing of omp gene
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Lee Yu-Ji
Kwon Ki-Tae
Park Mi-Yeoun
Peck Kyong-Ran
Song Jae-Hoon
Lee Nam-Yong
Oh Won-Sup
Ko Kwan-Soo
Ryu Seong-Yeol
Heo Sang-Taek
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Abstract
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Background : As the incidence of bovine brucellosis increases in Korea, the incidence of human brucellosis is also increasing since 2002. However, it is difficult to identify Brucella species by using the conventional methods.
Methods : Three strains of gram-negative coccobacilli were isolated from blood specimens of three patients with prolonged fever, which were not identified by using the conventional methods. After extracting total DNA from these isolates, PCR amplification of 16S rRNA and omp2 genes was performed. These sequences secured by PCR assay were compared with known sequences by using GenBank BLAST.
Results : DNA sequences were obtained from 3 isolates by using PCR amplification of 16S rRNA. These sequences had more than 99.9% similarities with Brucella species by using GenBank BLAST. In the second place, after comparing DNA sequences secured by PCR amplification of omp2a and omp2b by using GenBank BLAST, these isolates were confirmed as B. abortus.
Conclusion : DNA sequence analysis is a rapid and accurate method for identification of uncommon microorganisms, such as Brucella species.
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KEYWORD
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Brucellosis, Brucella arbortus, 16S ribosomal RNA, omp2
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